Inoculation methods | Max. biomass (g/L) | Max. rapamycin (mg/L) | Rapamycin content (mg/g cells) | Rapamycin productivity (mg/L h) |
---|---|---|---|---|
Liquid seed mediuma | ||||
2 % | 8.8 | 229 | 26 ± 3 | 1.91 |
5 % | 9.1 | 400 | 41 ± 3 | 3.04 |
10 % | 7.2 | 400 | 56 ± 5 | 3.71 |
Spore suspensionb | ||||
Low SS | 8.4 | 277 | 33 ± 2 | 1.92 |
High SS | 10.4 | 285 | 28 ± 3 | 1.83 |
Mycelium-barley | 14.4 | 520 | 36 ± 3 | 3.61 |