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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Sequence similarity network analysis, crystallization, and X-ray crystallographic analysis of the lactate metabolism regulator LldR from Pseudomonas aeruginosa

Fig. 5

a SDS-PAGE analysis of the purified recombinant PLldR protein. Lane M, protein molecular weight marker (labeled in kDa). Lanes 1 and 2, supernatant and pellet fractions after the centrifugation of the whole cell extract, respectively; lane 3, flow through from the Ni2+-affinity chromatography; lanes 4–7, the washing fractions with 20, 50, 110, and 170 mM imidazole, respectively; and lane 8, the final purified protein after the gel-filtration chromatography step. Samples of lane 8 were collected and concentrated to 10 mg mL−1 for crystal screening. b Single crystal of the PLldR protein from P. aeruginosa. The maximum dimensions of the crystals are 0.35 × 0.05 × 0.05 mm

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