Table 1 Protein production information
Source organism | Pseudomonas aeruginosa |
|---|---|
DNA source | Genomic DNA |
Forward primer | CATATGATGGAATTTGGTCAGGTCAG |
Reverse primer | GAATTCTTAGTCTTCCTGCACGCTG |
Cloning vector | pEASY-Blunt |
Expression vector | pET28a (+) |
Expression host | Escherichia coli BL21 (DE3) |
Complete amino acid sequence of the construct produced | MGSSHHHHHHSSGLVPRGSHMEFGQVRQRRLSDDIVAQLEAMILEGTLKSGERLPAERVLAEQFGVSRPSLREAIQKLVAKGLLVSRQGGGNYVTESLGATFSDPLLHLLEGNPEAQRDLLEFRHTLEGSCAYYAALRATSLDHQRLTEAFEALQACYARNDQVSAEEGAADARFHLAIAEASHNTVLLHTIKGLFDLLRRNVVTNIGGMYAQRTETRAQLMEQHQRLYDAIISGQAELAREVSNQHIHYVQEVLAEVQEEARRMKRSQRRRSVQED |