Table 2 Crystallization information

Plate type

24-well hanging-drop plate (Hampton research)

Temperature (K)

287

Protein concentration (mg mL⁻¹)

10

Buffer composition of protein solution

25 mM Tris–HCl, pH 8.0, 300 mM NaCl, and 2 mM dithiothreitol

Composition of reservoir solution

2 % w/v PEG 8000, 0.1 M Tris–HCl, pH 8.5

Volume and ratio of drop (µL)

1.0:1.0

Volume of reservoir (µL)

160