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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775

Fig. 4

Cell dimorphism reviled by online flow cytometry and optic microscopy. a Cell dimorphism occurring during a culture of Y. lipolytica JMY 775 in bioreactor and detected with online flow cytometric process. After gating two sub-populations, different morphologies can be distinguished, i.e., a1 elongated shape cell sub-population, a2 both morphotypes can co-exist in the same culture, and a3 single-cell shape sub-population. Analyses have been carried out with online FC and results were displayed on Forward Scatter Air/Forward Scatter Height (FSC-A/FSC-H) dot-plot on the basis of 40,000 events. b Optic microscopy images (40×), corresponding to samples of the panel “a”

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