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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker

Fig. 1

Schedule for the deletion of dal using Cre/lox system: (1) the front and back regions flanking the target gene to be deleted were PCR amplified, gel purified, and fused by PCR. The fragment lox71-zeo-lox66 was cloned from the plasmid p7Z6. (2) PCR-fused products were directly used to transform B. subtilis, and Zeor transformants were selected. (3) p148-cre was introduced into a Zeor clone, and the recombination between lox71 and lox66 was mediated by expressed Cre recombinase. (4) p148-cre was eliminated to get the target strain by 5 times sub-cultivation

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