From: Enhancement of asymmetric bioreduction of N,N-dimethyl-3-keto-3-(2-thienyl)-1-propanamine to corresponding (S)-enantiomer by fusion of carbonyl reductase and glucose dehydrogenase
Substrate
V max (μmol min−1 mg−1)
K m (mmol L−1)
k cat (s−1)
k cat/K m (L mol−1 s−1)
DKTP
6.75 ± 0.15
0.32 ± 0.05
4.35 ± 0.07
1.37 × 104
Glucose
2.58 ± 0.12
0.47 ± 0.04
1.66 ± 0.12
3.54 × 103