Fig. 1

Optimization of the temperature (a) and pH (b) of Module 2. Reaction conditions (0.5 mL): (−)-Perillyl alcohol 10 mM, LkADH 0.2 U/mL, [LkADH]/[NOX]/[ATA-117] = 1/5/10, 0.2 mM NAD⁺, 0.1 mM PLP, 50 mM 2-pentanamine, potassium phosphate buffer (pH 7.5, 100 mM) or Tris–HCl buffer (pH 8–9, 100 mM) or Gly-NaOH buffer (pH 9.5–10, 100 mM), 30℃, 35 ℃ or 40 ℃, 800 rpm, 12 h. Temp.: temperature; Concn.: concentration