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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: IRES-mediated Pichia pastoris cell-free protein synthesis

Fig. 3

Transcription optimization, screening of extract contents, and investigation of reaction time. A Schematic diagram of RI promotion for yield enhancement. B Effects of RI addition on protein expression. C Optimization of the extract volume. D Investigation of CFPS reaction time. The values in the graph are the mean values of three independent experiments, and the error line represents the standard deviation

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