Fig. 6
From: IRES-mediated Pichia pastoris cell-free protein synthesis
P. pastoris CFPS after design and optimization. A P. pastoris CFPS after the design and optimization. The components of the CFPS system, including extracts, DNA, energy, and reaction conditions, were optimized, and the protein synthesis reactions were promoted from two levels of transcription and translation. The red font in the figure shows the various factors affecting the reaction components and the reaction environment that were investigated in this experiment. B The comparison of the optimized P. pastoris CFPS system with the initial one. Based on the quantitative analysis (Additional file 1: Fig. S4), the sfGFP protein yield of the optimized system could reach 0.3Â mg/mL