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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene

Fig. 3

Overexpression and multi-copy integration of the mevalonate pathway genes for enhanced germacrene A biosynthesis. The increase in the copy number of TEF1p-ERG20-(PT)4P-GAS-AOX1t enhanced metabolic fluxes towards germacrene A (orange module). Overexpression and multi-copy integration of IDI1 and tHMG1 of the MVA pathway increased the titer of germacrene A (blue module). Overexpression of ACS increased the supply of acetyl-CoA and accordingly the titer of germacrene A (green module). "+" indicates pathway gene overexpression, and the corresponding number indicates the copy number of the pathway gene expression cassettes. The data represent average ± standard deviations of three biological replicates

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