Table 2 P. pastoris strains constructed in this study
Strain | Genotype | Source |
|---|---|---|
GAS0 | P. pastoris GS115-HIS4::Cas9 | |
GAS1 | GAS0-Int1::TEF1p-GAS-AOX1t | This study |
GAS2 | GAS0-Int1::TEF1p-ERG20-AOX1t-GAPp-GAS-CYC1t | This study |
GAS3 | GAS0-Int1::TEF1p-ERG20-GGS-GAS-AOX1t | This study |
GAS4 | GAS0-Int1::TEF1p-ERG20-GGGGS-GAS-AOX1t | This study |
GAS5 | GAS0-Int1::TEF1p-ERG20-(PT)4P-GAS-AOX1t | This study |
GAS6 | GAS0-Int1::TEF1p-ERG20-(PA)5-GAS-AOX1t | This study |
GAS7 | GAS5-Int12::TEF1p-ERG20-(PT)4P-GAS-AOX1t | This study |
GAS8 | GAS5-Int32::TEF1p-ERG20-(PT)4P-GAS-AOX1t-GAPp-ERG20-(PT)4P-GAS-CYC1t | This study |
GAS9 | GAS7-Int33::TEF1p-ERG20-(PT)4P-GAS-AOX1t-GAPp-ERG20-(PT)4P-GAS-CYC1t | This study |
GAS10 | GAS8-Int34::TEF1p-ERG20-(PT)4P-GAS-AOX1t-GAPp-ERG20-(PT)4P-GAS-CYC1t | This study |
GAS11 | GAS9-Int20::TEF1p-IDI1-AOX1t | This study |
GAS12 | GAS9-Int20::TEF1p-tHMG1-AOX1t | This study |
GAS13 | GAS9-Int20::TEF1p-IDI1-AOX1t-GAPp-tHMG1-CYC1t | This study |
GAS14 | GAS13-Int21::TEF1p-IDI1-AOX1t-GAPp-tHMG1-CYC1t | This study |
GAS15 | GAS14-Int31::TEF1p-IDI1-AOX1t-GAPp-tHMG1-CYC1t | This study |
GAS16 | GAS14-Int11::TEF1p-ACS-AOX1t | This study |
GAS17 | GAS16-Int16::TEF1p-ACS-AOX1t | This study |
GAS18 | GAS17-Int6::TEF1p-ACS-AOX1t | This study |