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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Overexpression of glutathione synthetase gene improving redox homeostasis and chicken infectious bursal disease virus propagation in chicken embryo fibroblast DF-1

Fig. 5

The effects of overexpression of gss gene on cellular redox homeostasis and anti-oxidative stress after IBDV infection in DF-1 cells. A Shows the fluorescence values at 488 nm of excitation wavelength and 525 nm of emission wavelength per million cells with 2,7-dichlorofluorescin diacetate treatment for the ROS detection at 0 hpi. B and C Show the intracellular concentration of GSH and GSSG in DF-1 cells at 0 hpi and 6 hpi of IBDV infection. D Shows the GSSG/GSH ratio calculated from B and C. E Shows the sod2 transcript level in DF-1 cells at 0 hpi and 6 hpi of IBDV infection. F and G Show the intracellular concentration of NADP+ and NADPH in DF-1 cells at 0 hpi and 6 hpi of IBDV infection. H Shows the NADP+/NADPH ratio calculated from F and G. N = 3 biological replicates and error bars represent s.d. Asterisks “*” presented the differences between the control cell and the recombinant overexpressing cells. *p < 0.05, **p < 0.01, and ***p < 0.001 as determined by two-tailed t test. “#” presented the differences between 0 and 6 hpi

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