Fig. 3

Effects of pH on the activity and stability of PaTreS with maltose as substrate. The 50 mM sodium acetate (pH 3.0–5.0), 50 mM potassium phosphate (pH 6.0–8.0), and 50 mM glycine–NaOH (pH 9.0–11.0) were used to assay the enzyme activities at various pH values (pH 4.0–11.0). To examine pH stability, the enzymes were incubated at various pH values (pH 4.0–11.0) at 30 °C for 1 h. The residual activities were measured at pH 7.0 using 1% (w/v) maltose as a substrate