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Table 5 Box–Behnken design

From: Optimization of tannase production by Aspergillus glaucus in solid-state fermentation of black tea waste

Run no.

X1: moisture ratio %

X2: initial pH

X3: substrate concentration

X4: sodium nitrate concentration

Y: tannase (U/mg)

Observed

Predicted

1

65

8

3

0.6

3.02

1.83125

2

65

8

6

1.0

2.94

3.27125

3

65

6

9

0.2

2.15

2.03417

4

65

4

9

0.6

2.15

2.56625

5

65

6

9

1.0

3.62

3.69750

6

100

6

6

1.0

2.32

1.96458

7

65

6

6

0.6

5.22

5.20333

8

30

6

6

0.2

0.42

0.00292

9

65

6

6

0.6

5.17

5.20333

10

65

4

3

0.6

2.37

2.14958

11

100

6

9

0.6

2.88

3.69125

12

30

4

6

0.6

1.32

1.77750

13

100

8

6

0.6

3.81

3.49750

14

65

4

6

1.0

3.33

2.79958

15

65

8

9

0.6

4.30

3.74792

16

65

6

3

0.2

1.79

1.85750

17

30

6

6

1.0

3.89

4.10625

18

100

6

3

0.6

1.48

2.74458

19

30

8

6

0.6

2.35

2.91417

20

30

6

3

0.6

1.42

1.23625

21

100

6

6

0.2

5.71

4.72125

22

65

8

6

0.2

1.40

2.55792

23

65

4

6

0.2

1.87

2.16625

24

100

4

6

0.6

4.19

3.77083

25

65

6

3

1.0

1.28

1.54083

26

30

6

9

0.6

3.26

2.62292

27

65

6

6

0.6

5.22

5.20333