Optimization of tannase production by Aspergillus glaucus in solid-state fermentation of black tea waste

Bioresources and Bioprocessing

Table 6 Analysis of variance

Source	DF	Adj SS	Adj MS	F value	P value
Model	14	43.4731	3.1052	4.30	0.002
Linear	4	10.9819	2.7455	3.80	0.032
Moisture%	1	4.9794	4.9794	6.89	0.022
pH	1	0.5590	0.5590	0.77	0.396
Substrate conc	1	4.0833	4.0833	5.65	0.035
NaNO₃	1	1.3601	1.3601	1.88	0.195
Square	4	18.6367	4.6592	6.44	0.005
Moisture%*moisture%	1	6.5318	6.5318	9.03	0.011
pH*pH	1	6.5318	6.5318	9.03	0.011
Substrate conc.*substrate conc	1	12.3695	12.3695	17.11	0.001
NaNO₃*NaNO₃	1	10.4222	10.4222	14.42	0.003
2-way interaction	6	13.8545	2.3091	3.19	0.041
Moisture%*pH	1	0.4970	0.4970	0.69	0.423
Moisture%*substrate conc	1	0.0484	0.0484	0.07	0.800
Moisture%*NaNO₃	1	11.7649	11.7649	16.27	0.002
pH*substrate conc	1	0.5625	0.5625	0.78	0.395
pH*NaNO₃	1	0.0016	0.0016	0.00	0.963
Substrate conc.*NaNO₃	1	0.9801	0.9801	1.36	0.267
Error		12	8.6756	0.7230
Lack-of-fit	10	8.6739	0.8674	1040.87	0.001
Pure error		2	0.0017	0.0008
Total		26	52.1487