Table 1 Strains used in this study
From: De novo synthesis of nervonic acid and optimization of metabolic regulation by Yarrowia lipolytica
Strains | Descriptions | Source |
|---|---|---|
E. coli JM109 | recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac-proAB)/F[traD36, proab+, lacIq, lacZΔM15] | Invitrogen |
Y. lipolytica Po1f | MatA, leu2-270, ura3-302, xpr2-322, axp1-2 | Nicaud (2012) |
GQY-∆PEX10 | Po1f-∆PEX10 | Gao et al. (2018) |
GQ06 | Po1f-∆PEX10 integrated MaELO3 by CRISPR/Cas9 at F1 site | Gao et al. (2020) |
NA01 | GQ06 integrated optimized AtKCS by CRISPR/Cas9 at A3 site | This study |
NA02 | NA01 integrated optimized CraKCS by CRISPR/Cas9 at F1-3 site | This study |
NA03 | NA01 integrated optimized CgKCS by CRISPR/Cas9 at AXP site | This study |
NA04 | NA03 integrated optimized CraKCS by CRISPR/Cas9 at F1-3 site | This study |
NA05 | NA04 cells harboring pINA1312-PUT- MaD15D | This study |
NA06 | NA04 cells harboring pINA1312-PUT- CsD15D | This study |
NA07 | NA04 integrated optimized CgKCS-L-MaD15D by CRISPR/Cas9 at A1-2 site | This study |
NA08 | NA07 integrated optimized CgKCS-L-MaD15D by CRISPR/Cas9 at E1-3 site | This study |
NA09 | NA04 cells harboring pINA1312-PUT- DGA1 | This study |
NA10 | NA08 cells harboring pINA1312-PUT- OLE1 | This study |
NA11 | NA08 cells harboring pINA1312-PUT- DGA1-L-OLE1 | This study |
NA12 | NA08 cells harboring pINA1312-PUT- OLE1-L-DGA1 | This study |
NA13 | NA12 cells harboring pINA1269- OLE1-L-DGA1 | This study |
NA14 | NA10 cells harboring pINA1269-DGA1 | This study |
NA15 | NA12 cells harboring pINA1269-DGA1 | This study |
NA16 | NA12 cells harboring pINA1269-MaELO3 | This study |
NA17 | NA12 cells harboring pINA1269-MaELO3-AtKCS | This study |
NA18 | NA12 cells harboring pINA1269-MaELO3-CraKCS | This study |
NA19 | NA12 cells harboring pINA1269-CgKCS | This study |
NA20 | NA04 cells harboring pINA1269-ACL | This study |
NA21 | NA04 cells harboring pINA1269-ACS2 | This study |
NA22 | NA04 cells harboring pINA1269-ACC1 | This study |
NA23 | NA20 cells harboring pINA1312-ACS2 | This study |
NA24 | NA04 integrated FAA1 by CRISPR/Cas9 at MFE site | This study |