Table 1 Box–Box–Behnken design (BBD) used in this research article
Independent variables | Coded independent variables | |||||
|---|---|---|---|---|---|---|
Run | Temperature (°C) × 1 | Extraction cycle time (min) × 2 | Solvent-to-biomass ratio (mL/mg) × 3 | Temperature (°C) × 1 | Extraction cycle time (min) × 2 | Solvent-to-biomass ratio (mL/mg) × 3 |
1 | 50 | 20 | 60 | − 1 | − 1 | 0 |
2 | 70 | 20 | 60 | 1 | − 1 | 0 |
3 | 50 | 30 | 60 | − 1 | 1 | 0 |
4 | 70 | 30 | 60 | 1 | 1 | 0 |
5 | 50 | 30 | 50 | − 1 | 0 | − 1 |
6 | 70 | 30 | 50 | 1 | 0 | − 1 |
7 | 50 | 30 | 70 | − 1 | 0 | 1 |
8 | 70 | 30 | 70 | 1 | 0 | 1 |
9 | 60 | 20 | 50 | 0 | − 1 | − 1 |
10 | 60 | 40 | 50 | 0 | 1 | − 1 |
11 | 60 | 20 | 70 | 0 | − 1 | 1 |
12 | 60 | 40 | 70 | 0 | 1 | 1 |
13 | 60 | 30 | 60 | 0 | 0 | 0 |
14 | 60 | 30 | 60 | 0 | 0 | 0 |
15 | 60 | 30 | 60 | 0 | 0 | 0 |