Fig. 2

Effect of amino donors of VfTA on the production of (+)-neomenthylamine. Reaction conditions (1 mL): 5 mM (−)-menthone, 2 mg/mL purified VfTA, 1 mM PLP, KPB buffer (100 mM pH 7.0), 1% DMSO, in addition to 50 mM amino donor of L-Ala, isopropylamine, isobutylamine, (S)-2-pentanamine, (S)-2-hexaneamine, (S)-2-heptanamine, (S)-2-nonanamine, (S)-α-methylbenzylamine or benzylamine. All mixtures were shaken at 30 °C, 1000 rpm for 24 h.