Fig. 3

Optimization of the system parameters of VfTA-catalyzed transamination. Reaction conditions (1 mL): 5 mM (−)-menthone, 50 mM (S)-α-methylbenzylamine, 2 mg/mL purified VfTA, 1 mM PLP, KPB buffer (100 mM pH 7.0), 1% DMSO, 30 °C, 1000 rpm for 24 h, unless otherwise noted. (A) citrate buffer (100 mM pH 4.0–6.0) or potassium phosphate buffer (100 mM pH 6.0–8.5) or Gly-NaOH buffer (100 mM pH 8.5–9.5); (B) incubation temperature: 25 °C, 30 °C, 35 °C, or 40 °C; (C) 10–70 mM (S)-α-methylbenzylamine; (D) 0.5–2 mM PLP.