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Bioresources and Bioprocessing

Table 2 Kinetic constants of VfTA toward substrate (−)-menthone and amino donor S-MBAa

From: Construction and optimization of a biocatalytic route for the synthesis of neomenthylamine from menthone

Entry

Compound

Vmax (U/mg)

KM (mM)

kcat (s−1)

kcat/KM (s−1 mM−1)

VfTA

(−)-Menthone

0.0054 ± 0.0002

5.0 ± 0.6

16.3 ± 0.0

3.3

(S)-α-Methylbenzylamine

0.014 ± 0.000

0.8 ± 0.1

42.3 ± 0.0

54.4

  1. aAll reactions were carried out in triplicates, and the average values with standard deviation were shown. The kinetic curves of VfTA for substrate and amino donor were fitted by Prism 9
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