Table 1 Summarized the use of multi-enzyme complexes based on SpyTag/SpyCatcher, cohesin–dockerin and SH3–PDZ–GBD for enzyme cascade
From: Research progress of multi-enzyme complexes based on the design of scaffold protein
Scaffold protein | Location | Function | References |
|---|---|---|---|
SpyTag/SpyCatcher | In vivo | Achieved higher sugar conversion yield | (Jia et al. 2017) |
In vivo | Increased the oil synthesis | (Zhong et al. 2022a) | |
In vivo | Improved the catalytic efficiency | ||
Cohesin–dockerin specificity | In vitro | Improved catalytic efficiency | (You 2012) |
In vitro | Increased the reaction rate of bioelectricity generation | (Meng et al. 2019) | |
E. coli cell surfaces | Enhanced the power density and operational stability | (Cai et al. 2022) | |
SH3, PDZ, GBD domain and ligand | In vivo | Increased the production of mevalonate | (Dueber et al. 2009) |
In vivo | Increased the production of GBGA | (Pham et al. 2016) |