Table 2 Characteristics of different types of linkers
From: Research progress of multi-enzyme complexes based on the design of scaffold protein
Linker | Fusion protein | Function | References | |
|---|---|---|---|---|
Type | Sequence | |||
Flexible linker | GGGGS | HSA–IFN-α2b | Improve biological activity | (Zhao et al. 2008) |
TD1–hEGF | Inhibit the permeation activity | (Ruan et al. 2014) | ||
(GGGGS)2 | DFR–LAR | Improve catalytic efficiency | (Sun et al. 2021) | |
(GGGGS)3 | IL-2–PfCelTOS | Enhance structural stability | (Shamriz et al. 2016) | |
Tax–gp21–gp46–gag | Increase expression level | (Kabiri et al. 2020) | ||
(GGGGS)4 | CotB–Tm1350 | Enhance enzyme activity | (Ullah et al. 2017) | |
CotB–DSM | Enhance enzyme activity and stability | (Chen et al. 2017) | ||
Rigid linker | EAAAK | CBDs–CP05 | Enhance structural stability | (Ma et al. 2022) |
Fluc–LRE | Improve stability and continuity | (Sun et al. 2018) | ||
(EAAAK)2 | DFR–LAR | Improve catalytic efficiency | (Sun et al. 2021) | |
(EAAAK)3 | Glu–Xyl | Increase catalytic efficiency | (Lu and Feng 2008) | |
(EAAAK)n (n = 2,3) | VP1–SpyTag | Enhance conjugation efficiency | (Boonyakida et al. 2023) | |
EAAAK (PAPAP)n (n = 1,2) | HSA–CocH1 | Prolong biological half-life | (Cai et al. 2019) | |