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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Iterative gene integration mediated by 26S rDNA and non-homologous end joining for the efficient production of lycopene in Yarrowia lipolytica

Fig. 3

Optimized expression of AtoB, HMGR, and ERG13 via non-homologous end joining (NHEJ)-mediated multi-round iterative transformation. A Schematic diagram of NHEJ-mediated multi-round iterative transformation, showing the color performance of each transformant on the plate; B lycopene production of transformants after expression of AtoB and HMGR via multi-round transformation. The red lines indicate the average yield of each round. C Lycopene production of different engineered strains; data are presented as the averages of two replicates with error bars representing standard deviations; “*” represents genes optimized via multi-round iterative transformation

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