Table 2 Box Behnken design along with experimental and predicted values of dependent variable for laccase production
Run | A: coconut husk (g/L) | B: CuSO4 (µM) | C: yeast extract (g/L) | D: pH | Laccase production (IU/mL) | |
|---|---|---|---|---|---|---|
Experimental | Predicted | |||||
1 | −1 | 0 | 1 | 0 | 21.8 | 23.35 |
2 | −1 | 0 | −1 | 0 | 46.7 | 46.08 |
3 | 0 | −1 | 0 | −1 | 38.1 | 37.58 |
4 | 0 | −1 | 1 | 0 | 24.4 | 23.85 |
5 | 1 | 0 | 1 | 0 | 13.5 | 14.95 |
6 | 0 | 1 | 0 | 1 | 26.5 | 27.85 |
7 | 0 | 0 | 0 | 0 | 52.4 | 51.38 |
8 | 1 | 0 | 0 | −1 | 38.7 | 36.71 |
9 | 0 | 0 | 0 | 0 | 51.7 | 51.38 |
10 | 1 | 1 | 0 | 0 | 17.4 | 17.52 |
11 | −1 | 0 | 0 | −1 | 36.5 | 36.96 |
12 | 0 | 0 | 0 | 0 | 50.3 | 51.38 |
13 | 0 | 0 | 0 | 0 | 50.1 | 51.38 |
14 | 0 | 0 | −1 | −1 | 49.2 | 50.2 |
15 | 0 | −1 | −1 | 0 | 40.8 | 41.43 |
16 | 0 | 1 | 0 | −1 | 38.3 | 39.32 |
17 | 0 | 1 | −1 | 0 | 44.1 | 44.66 |
18 | 0 | −1 | 0 | 1 | 32.7 | 32.52 |
19 | −1 | −1 | 0 | 0 | 28.8 | 27.84 |
20 | 1 | −1 | 0 | 0 | 32.1 | 33.69 |
21 | 0 | 0 | 1 | 1 | 21.5 | 19.65 |
22 | 1 | 0 | 0 | 1 | 20.3 | 19.85 |
23 | 0 | 0 | 0 | 0 | 52.4 | 51.38 |
24 | 0 | 1 | 1 | 0 | 18.3 | 17.68 |
25 | −1 | 1 | 0 | 0 | 43.5 | 41.07 |
26 | 0 | 0 | 1 | −1 | 27.2 | 27.22 |
27 | −1 | 0 | 0 | 1 | 35.3 | 37.3 |
28 | 0 | 0 | −1 | 1 | 42.1 | 41.24 |
29 | 1 | 0 | −1 | 0 | 37.5 | 36.78 |