Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Bioresources and Bioprocessing

Table 3 ANOVA for laccase production as a function of independent variables

Source	Sum of squares	df	Mean Square	F value	p value Probe > F
Model	3839.97	14	274.28	101.52	< 0.0001*
A-coconut husk	234.97	1	234.97	86.97	< 0.0001*
B-CuSO₄	6.45	1	6.45	2.39	0.1445
C-yeast extract	1489.64	1	1489.64	551.37	< 0.0001*
D-pH	205.01	1	205.01	75.88	< 0.0001*
AB	216.09	1	216.09	79.98	< 0.0001*
AC	0.2025	1	0.2025	0.075	0.7883
AD	73.96	1	73.96	27.38	0.0001*
BC	22.09	1	22.09	8.18	0.0126*
BD	10.24	1	10.24	3.79	0.0719
CD	0.49	1	0.49	0.1814	0.6767
A²	855.1	1	855.1	316.51	< 0.0001*
B²	631.79	1	631.79	233.85	< 0.0001*
C²	598.63	1	598.63	221.57	< 0.0001*
D²	335.71	1	335.71	124.26	< 0.0001*
Residual	37.82	14	2.7
Lack of fit	32.84	10	3.28	2.63	0.1818^ns
Pure error	4.99	4	1.25
Cor Total	3877.8	28