Fig. 2

Effect of O. anisum root extract in HCl-induced ALI mice. Different doses ranging from 50 to 200 mg/kg were assessed in ALI mice. Animals subjected to HCl served as positive control, while animals received only food and water served as negative control. A Total number of cells from BALF of animals treated with and without O. anisum extract. B Total number of neutrophils from BALF in the presence and absence of O. anisum extract. C Total protein content from BALF of animals treated with and without O. anisum extract. D ROS level in the presence and absence of the O. anisum. E MDA levels of animals treated with and without O. anisum extract. F The reduced GSH levels of animals treated with and without the extracts in HCl-induced ALI mice. G The levels of redox homeostasis in the presence and absence of O. anisum extract. H Catalase activity in the presence and absence of O. anisum extract. I O. anisum extract down regulates the expression of NFkB-associated genes in the lungs of HCl-induced mice. The lung tissue was collected from the positive control (HCl exposed) mice and O. anisum extract treated mice. Total RNA was isolated and purified from the control and treated lung samples and converted into cDNA. The cDNA was assessed for the pro-inflammatory associated gene expression (IL-1β, TNF-α, and ICAM-1) using their respective primers. β-actin, a reference gene, used to normalize the gene expression. Data are denoted as mean ± SD (n = 6)