Fig. 3

Western blots and fluorescent microscopy of immunostained E. coli cells. A Western blots of the immunoprecipitants from cell membrane fractions of E. coli co-display-cp52k (lane 1); E. coli co-display-mfp (lane 2) and E. coli sole-display (lane 3). The bands of mouse IgG heavy chain are indicated. B Western blots of cell membrane proteins for E. coli co-display-mfp (lane 1) and E. coli co-display-cp52k (lane 2). C Fluorescent microscopy of immunostained E. coli cells solely expressing Fast-PETase or adhesive protein on the surface and D E. coli cells simultaneously expressing Fast-PETase and adhesive protein on their surface. Scale bar = 10 μm