Fig. 4
From: Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate
Quantification of Fast-PETase on the surface of engineered E. coli cells and PET degradation rates of engineered E. coli systems. (A) PETase-grayscale analysis of approximately 60 ng, 50 ng, 40 ng and 30 ng Fast-PETase by Western blotting. B Grayscale-protein concentration standard curve. The values represent the mean ± SD. C PETase-grayscale analysis of cell membrane proteins of E. coli strains with AIDA-PETase (sole-display), co-display-cp52k and co-display-mfp by Western blotting. Each lane contained cell membrane proteins from approximately 5.4 mg E. coli cells (wet weight). The samples of the same experiments were processed in parallel. D Comparison of PET degradation rate (%) for three E. coli cell systems and free Fast-PETase. All experiments were conducted in triplicate. The values are expressed as mean ± SD, and the asterisks denote the statistically significant difference (p < 0.01, unpaired t-test)