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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

Fig. 1

NcAA incorporation efficiency comparison between MmPylRS (N346A/C348A) and MbPylRS (N311A/C313A). A The chemical structures of 12 ncAAs used for testing incorporation efficiency. 1, 3-iodo-L-Phe (3-I-Phe); 2, 3-bromo-L-Phe (3-Br-Phe); 3, 3-methyl-L-Phe (3-Me-Phe); 4, 3-chloro-L-Phe (3-Cl-Phe); 5, 2-trifluoromethyl-L-Phe (2-CF3-Phe); 6, 3-nitro-L-Phe; 7, O-benzyl-L-tyrosine; 8, O-tert-butyl-L-tyrosine; 9, O-methyl-L-tyrosine; 10, 2-methyl-L-Phe (2-Me-Phe); 11, 2-bromo-L-Phe (2-Br-Phe); 12, 2-chloro-L-Phe (2-Cl-Phe). B Activity of Mm-NA/CA and Mb-NA/CA toward different ncAAs. Translation of the sfGFP reporter (UAG codon at position 2) by the two PylRS variants was measured by fluorescence intensity

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