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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

Fig. 2

Incorporation efficiency of PylRS mutants toward 8 ncAAs during greedy evolution. A Incorporation efficiency of single mutants. Avg refers to the mutants’ average incorporation efficiency for the 8 ncAAs. B Incorporation efficiency of double mutants starting from T56P. C Incorporation efficiency of triple mutants starting from V31I/T56P. D Incorporation efficiency of quadruple mutants starting from V31I/T56P/A100E. E Comparison of incorporation efficiency between the optimal mutant obtained from each round of greedy evolution and IPYE, EIVLRT and Mb-NA/CA

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