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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

Fig. 3

Analysis of MbPylRS complex structure bound with tRNAPyl. A Superposition of the MbPylRS-tRNAPyl complex predicted by AlphaFold2. B N-terminal residues selected for greedy evolution are represented as sticks, with V31, T56 and A100 represented as spheres. The secondary structure features of tRNAPyl are colored: blue, D loop; brown, anticodon stem; purple, anticodon; yellow, variable loop; orange, T loop; green, acceptor stem. C Close-up view of T56 and the surrounding residues. D Close-up view of V31 and the surrounding residues

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