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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

Fig. 4

Molecular structures of the 16 novel ncAAs in four classes accepted by IPE variant and the incorporation efficiency of Mb-NA/CA and IPE toward these ncAAs. Structures of A halogenated Phes, B thienyl-L-alanines, C non-phenyl-substituted Phes, D para-substituted Phes. Incorporation efficiency of Mb-NA/CA and IPE toward E halogenated Phes, F thienyl-L-alanines, G non-phenyl-substituted Phes, H para-substituted Phes. 13, L-2,5-dichloroPhe; 14, L-2,4-difluoroPhe; 15, L-2,3-difluoroPhe; 16, L-2,5-difluoroPhe; 17, L-2,4,5-trifluoroPhe; 18, L-2,3,6-trifluoroPhe; 19, L-2,3-dichloroPhe; 20, 5-bromo-2-chloro-L-Phe; 21, L-2-(5-bromothienyl)alanine; 22, L-3-benzothienylalanine; 23, 3-(3-thienyl)-L-alanine; 24, 3-(2-thienyl)-L-alanine; 25, Phenyl-lactic acid; 26, 2-amino-2-phenylpropionic acid; 27, L-homoPhe; 28, 4-nitro-L-Phe

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