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Bioresources and Bioprocessing
Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

Fig. 6

Incorporation of 8 ncAAs into the 412 site of PedH. A Crystal structure of PedH (PDB ID 6ZCW) and close-up view around the 412 site of PedH. The light blue channel indicates the substrate channel predicted by Caver 3. B Structures of the 8 ncAAs incorporated into PedH. C Specific activities of the variants toward ethanol and methanol. D Specific activities of the variants toward (S)-2-butanol and (R)-2-butanol

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