Bioresources and Bioprocessing

Table 1 Mass spectra to investigate incorporation of 16 ncAAs by Mb-NA/CA-IPE

From: An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

ncAA	Expected^a (Da)	Observed (Da)
13	27856.89	27857.38
14	27823.98	27788.42, 27826.84
15	27823.98	27824.63
16	27823.98	27788.46, 27825.32
17	27841.97	27842.54
18	27841.97	27842.36
19	27856.89	27856.99
20	27901.34	27901.85
21	27872.92	27873.39
22	27844.09	27844.63
23	27794.03	27792.26
24	27794.03	27790.66
25	27788.98	27788.49
26	27788.0	27788.81
27	27802.03	27769.88, 27788.70, 27805.78
28	27833.0	27788.76, 27831.27

^aLC–MS expected mass of sfGFP-S2TAG with ncAAs incorporated

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