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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification

Fig. 3

Elution profiles of Molecule A (top); Molecule B (middle); and Molecule C (bottom) from CHT phosphate gradient elution (CHT-P, left column); CHT-NaCl gradient elution (CHT-NaCl, middle column); and CEX (right column). Each plot demonstrates an overlay of chromatogram (A280) with protein mass in each elution fraction (bar graph), comprising Main (white); HMW (red); and LMW (black). The x-axis demonstrates volume during an elution phase (40 CV). Dotted lines demonstrate conductivity (mS/cm), required for corresponding elution fractions. Red boxes indicate elution fractions, containing mainly LMW species

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