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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification

Fig. 5

Non-reducing SDS-PAGEs, illustrating protein populations in HCCF, Post Protein A, Post CHT-P (top), Post CHT-NaCl (bottom), and early elution fractions prior to main product peaks (Fx) of Molecule A (left column); Molecule B (middle column); and Molecule C (right column), respectively. Marker units are in kDa. Protein populations, indicated as (*), were proposed as suggested by molecular weights, which were calculated from amino acid sequences. Abbreviations used are listed as follows; M = monomer; HH = hole–hole homodimer; KK = knob–knob homodimer; LC = light chain; w/o = without. Existence of large LMWs in early elution fractions indicated early elution of large LMWs, as compared to monomers

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