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Bioresources and Bioprocessing
Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification

Fig. 6

Bar graphs, illustrating %yield (left); and “chromatography-induced aggregation (right) of Molecules A, B, and C after CHT sodium chloride gradient elution (CHT-NaCl), CHT phosphate gradient elution (CHT-P), and CEX. Levels of “chromatography-induced aggregation”, was defined by the ratios of [HMW contents in all elution fractions] over [HMW contents in loads]. HMW contents in the fractionated elutes during the gradient elution and that in CEX and CHT loads were calculated based on SEC-HPLC analysis to generate the ratios for evaluation. Higher ratios indicated more aggregates were generated during chromatography

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