Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification

Bioresources and Bioprocessing

Table 3 C-site binding ratios, pI values, and C-site binding preference for scFv, Fab, Fc domain compositions for bsAbs Molecules A, B, and C

bsAb Domain	C-site binding ratio	pI value	C-site binding preference
scFv	0.70	9.09	Less favoured
Fab	0.85	8.64	Less favoured
Fc (knob)	1.0	6.94	More favoured
Fc (hole)	1.0	6.94
Fc (wild type)	1.0	7.18

Fc (knob) and Fc (hole) are Fc domains of Molecules A and B, while Fc (wild type) is the Fc domain for Molecule C