Skip to main content
Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Bioengineering Comamonas testosteroni CNB-1: a robust whole-cell biocatalyst for efficient PET microplastic degradation

Fig. 2

Heterogeneous expression of DuraPETase was observed in C. testosteroni CNB-1. A DuraPETase was fused with different signal peptide and promoters to facilitate extracellular secretion in CNB-1. Different recombinant plasmids were constructed and introduced into CNB-1 to generate corresponding strains, which were designated as CNB-1A to CNB-1D, respectively. B The secretion efficiency of DuraPETase with or without OmpC signal peptide, was determined by measuring the intensity of extracellular GFP, which was fused with DuraPETase. C The hydrolytic activity of the recombinant strains was assessed on Impranil® DLN plates

Back to article page