Fig.1From: Expression of chitosanase from Aspergillus fumigatus chitosanase in Saccharomyces cerevisiae by CRISPR-Cas9 toolsAnalysis of DNA gel electrophoresis for colony PCR fragments. Lane M, DNA marker; Lanes 1–5: Validation of X-2 site; Lanes 6–10: Validation of insertion at XII-2 site; Bands appear in both Lane 4 and Lane 9, indicating that the strain in question is a biallelic yeast strainBack to article page