Fig. 2
From: Enzyme engineering for functional lipids synthesis: recent advance and perspective
Schematic representation of rational design to reconstruct the substrate pocket for improved PLD activity in phosphatidylserine production. The PLD structure depicted in a, b, c was modeled utilizing SWISS MODEL, utilizing the template protein from Protein Data Bank (PDB) ID: 1f0i and visualized using PyMOL; AutoDock Vina was employed to perform docking of PLD in complex with the substrate as shown in (a), with subsequent analysis conducted using the PLIP server; c Experimental assessment of enzyme activity and phosphatidylserine synthesis on a 3-liter scale; d Hydrophobic cavity computed with the POCASA server, indicating changes before and after mutations, implying an expanded substrate pocket for better substrate accommodation as one of the reasons for enhanced activity. Detailed experimental results are provided in the reference (Qi et al. 2022)