Fig. 2From: A circuitous route for in vitro multi-enzyme cascade production of cytidine triphosphate to overcome the thermodynamic bottleneckEffect of pH (A) and temperature (B) on CDAs. The effect of pH was determined at 50 °C in buffers with different pH values. The effect of temperature was determined in PB buffer (pH 7.0) at pH 5.0. The reaction system consisted of 10 mM cytidine, 5 μmol of various pure enzymes. All measurements were carried out in triplicatesBack to article page