Fig. 3From: A circuitous route for in vitro multi-enzyme cascade production of cytidine triphosphate to overcome the thermodynamic bottleneckKinetic results of wild-type (A) and the variant (B) on ATP in the presence of different concentrations of CTP. Kinetic results of wild-type (C) and the variant (D) on UTP in the presence of different concentrations of CTP. Fitting was performed using GraphPad software, and the allosteric sigmoidal model was selected. All measurements were carried out in triplicatesBack to article page