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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: A circuitous route for in vitro multi-enzyme cascade production of cytidine triphosphate to overcome the thermodynamic bottleneck

Fig. 5

Two-stage synthesis of CTP using 20 mM cytidine as substrate. The reaction was initiated at 50 °C in 50 mM PB buffer (pH 5.0) containing 2 mM ATP, 5 mM polyP6, 20 mM MgCl2, and pure enzyme to generate UMP. After 2.0 h of the first-stage reaction, the pH and temperature of the solution were adjusted, and the remaining enzyme and substrate were added to produce CTP. All measurements were carried out in triplicates

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