A circuitous route for in vitro multi-enzyme cascade production of cytidine triphosphate to overcome the thermodynamic bottleneck

Bioresources and Bioprocessing

Table 1 Key information about the enzymes used in this study

Enzyme name	Microorganism source	Specific activity (U/mg)	Refs.
Cytidine deaminase	Sulfurovum riftiae	38.8 ± 0.4	This article
Nucleoside kinase^a	Phorcysia thermohydrogeniphila	36.5 ± 0.7	Li et al. (2022)
UMP kinase^b	Sulfurovum lithotrophicum	37.1 ± 1.1	Li et al. (2022)
Nucleoside diphosphate kinase	Desulfurella amilsii	450.9 ± 4.3	Li et al. (2022)
CTP synthase^c	Aquifex aeolicus	2.7 ± 0.1	This article
Polyphosphate kinase	Sulfurovum lithotrophicum	180.5 ± 2.6	Sun et al. (2021)