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Bioresources and Bioprocessing

Table 2 The specific activity of AaCTPS and the seven variants

From: A circuitous route for in vitro multi-enzyme cascade production of cytidine triphosphate to overcome the thermodynamic bottleneck

Enzyme

Specific activity (U/mg)

WT

1.55 ± 0.04

I19T/R34G/R262H

2.68 ± 0.07

I19T

2.32 ± 0.09

R34G

1.73 ± 0.01

R262H

1.56 ± 0.02

I19T/R34G

1.20 ± 0.03

I19T/R262H

2.07 ± 0.10

R34G/R262H

1.93 ± 0.06

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