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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Biotransformation of 2-keto-4-hydroxybutyrate via aldol condensation using an efficient and thermostable carboligase from Deinococcus radiodurans

Fig. 2

Effects of pH, temperature, and metal ions on MBP-DrADL activity. A Effect of pH. The reactions were performed using three different types of buffers: PIPES (pH 6.5–7.5), EPPS (pH 7.5–8.5), and CHES (pH 8.6–10.0). B Effect of temperature. C Thermostability of MBP-DrADL. The reactions were performed with 0.002 mg mL−1 enzyme, 5 mM formaldehyde, and 5 mM pyruvate in the presence of 1 mM Mg2+ for 10 min. D Effect of different metal ions. E Effect of Mg2+ concentration. The reactions were conducted in 50 mM EPPS (pH 8.0) buffer containing 0.002 mg mL−1 enzyme, 5 mM formaldehyde, and 5 mM pyruvate at 50 °C for 10 min

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