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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Biotransformation of 2-keto-4-hydroxybutyrate via aldol condensation using an efficient and thermostable carboligase from Deinococcus radiodurans

Fig. 4

High production of 2-KHB from formaldehyde and pyruvate using MBP-DrADL. A Time-course reactions without batch-feeding substrates. The reactions were performed in 50 mM EPPS (pH 8.0) buffer containing 0.025 mg mL−1 enzyme, 100 mM formaldehyde, 200 mM pyruvate, and 5 mM Mg2+ at 50 °C for 1 h. B Time-course reactions while batch-feeding substrates. The reactions were performed under the same condition as A, but 50 mM formaldehyde and 50 mM pyruvate were added every 10 min up to 60 min

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