Biotransformation of 2-keto-4-hydroxybutyrate via aldol condensation using an efficient and thermostable carboligase from Deinococcus radiodurans

Bioresources and Bioprocessing

Table 2 Quantitative 2-KHB production as the main product from formaldehyde and pyruvate by pyruvate aldolases

Enzyme	Substrate [mM]	Product [mM] (g L^–1)	Volumetric productivity (g L^–1 h^–1)	Specific productivity (mg mg-enzyme^–1 h^–1)	References
MBP-EcYfaU	Formaldehyde [1000] Pyruvate [1000]	[1196] (140)	5.83	5.83	Hernandez et al. (2017)
MBP-PaADL^WT	Formaldehyde [100] Pyruvate [200]	[51.6] (6.03)	24.2	334.0	Jeong et al. (2023)
MBP-PaADL^V121A/L241A	Formaldehyde [100] Pyruvate [200]	[73.6] (8.60)	34.5	334.6	Jeong et al. (2023)
MBP-DrADL	Formaldehyde [100] Pyruvate [200]	[76.5] (8.94)	8.94	357.6	This study
MBP-DrADL^a	Formaldehyde [100] Pyruvate [200]	[124.8] (14.6)	14.6	583.4	This study